2 edition of Inheritance, expression and screening of reporter genes in transgenic zebrafish found in the catalog.
Inheritance, expression and screening of reporter genes in transgenic zebrafish
Patrick David Lyon Gibbs
Written in English
|Statement||by Patrick David Lyon Gibbs.|
|The Physical Object|
|Pagination||vii, 114 leaves, bound :|
|Number of Pages||114|
Zebrafish (Danio rerio) possesses the physiology complexity of mammalian animals and is one of the mostcommon vertebrate model ve Biostructure establishes and provides the MagHelix™ Zebrafish Screening Platforms for scientific and medical research. Zebrafish can be used to study vertebrate development and gene function. On the other hand, it is also an excellent choice to. To follow the expression of this gene in live embryos, we created transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of lhx1a regulatory regions. Tg(lhx1a:EGFP) (pt) recapitulates the expression of endogenous lhx1a beginning at early gastrula stages through 72 hours of development with only few.
Combinatorial genetics for conditional transgene activation allows studying gene function with temporal and tissue specific control like the Gal4-UAS system, which has enabled sophisticated genetic studies in Drosophila. Recently this system was adapted for zebrafish and promising applications have been introduced. Here, we report a systematic optimization of zebrafish Gal4-UAS genetics by Cited by: The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides information about when genes are active in developing embryos.
In either case, it is important to create transgenic zebrafish with specific and strong Gal4 expression. For UAS reporter and effector fish to function successfully, it is important to generate lines carrying at least 10 insertions of the UAS-reporter or UAS-effector construct and select the one that works best with the Gal4 transgenic fish of interest. The results suggest that non-homologous end joining (NHEJ) was the principal mechanism of exogenous gene concatemer formation and integration of transgenes into the genome of transgenic zebrafish. Keywords: Transgene, Concatemer, DSB (double strand breaks), NHEJ (non-homologous end joining), ZebrafishCited by:
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In these transgenic embryos, the expression domains of the reporter d2EGFP have not fully recapitulated all the anatomical regions (such as somites and tail bud) where a known FGF target, the gene dusp6, is normally by: Generation of transgenic FGF reporter lines.
The zebrafish Dusp6 gene locus was identified by PCR screening a BAC library from Genome Systems. The BAC clone contained the full gene Dusp6 locus, and a 10 Kb fragment that includes the 5' untranslated sequence within exon I was subcloned into a vector containing the d2EGFP, a gene that encodes a destabilised green fluorescent Cited by: Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.
Abstract. In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of by: 5. We carried out a detailed time course characterization of reporter gene expression to test the utility of SB as a permanent transgenic marker in zebrafish.
To follow the GFP expression under the EF1α enhancer/promoter (pT/XEX-GM2′2), embryos collected from homozygous male and female outcrosses were systematically examined for GFP expression Cited by: Another reporter fish model, the transgenic cyp19a1b-GFP zebrafish, expresses GFP under the control of the ERregulated cyp19a1b (brain aromatase) gene in radial glial cells in the brain).
Transgenic animals with reporter gene expression in specific tissues or cell types are valuable experimental tools.
In zebrafish, an important model organism in Cited by: This technology has been used in zebrafish to enable the studies of gene function at the molecular level as well as for drug screening. The transgenic zebrafish line Tg(7:nlsGFP) that expresses enhanced green fluorescent protein (eGFP) in bone has already been used by researchers to study the development of axial skeleton.
Our study demonstrates the feasibility of monitoring expression of multiple genes in different tissues in the same transgenic organism.
Two tissue-specific promoters were used to express both green fluorescent protein (GFP) and red fluorescent protein (RFP) in transgenic zebrafish by: As zebrafish are a well validated model for genetic studies and developmental biology, our objective was to establish the spatiotemporal expression pattern of a number of OA susceptibility genes in the larval zebrafish providing a platform for functional studies into the role of these genes in by: Fluorescent reporter genes are used to visualize biological processes in zebrafish and have been used successfully in genetic screens to identify mutants (for Cited by: The Zebrafish Book; Protocol Wiki; Antibody Wiki; Anatomy Atlases; Resources for Students and Educators; Search for Gene Expression Data.
Gene/EST: Fish: Target Gene: Author: Show only reporter genes in transgenic fish. Show only figures with images. Show only direct submission data. Show only published literature. DNA constructs for proper gene expression was laborious with standard molecular manipulations, since researchers had to make a lot of constructs, and had to test the enhancer activity of each construct.
If we can place reporter genes into BACs, the labor-intense steps can largely be bypassed. This can nowCited by: Zebrafish chemical screen identifies an FGF modulator. We previously described the generation of a transgenic zebrafish line, Tg(dusp6:EGFP) pt6, that Cited by: In this study, we used a multicopy UAS and fluorescent reporter genes to monitor transcriptional silencing in transgenic zebrafish.
Independent GFP high, GFP low, and GFP off epiallelic lines were established, which allowed silencing of gfp expression to be followed in developing tissues and over multiple by: Generation of Two-color Transgenic Zebrafish Using the Green and Red Fluorescent Protein Reporter Genes gfp and rfp Haiyan Wan, Jiangyan He, Bensheng Ju, Tie Yan, Toong Jin Lam, and Zhiyuan Gong*.
First, the chemically induced mutant genes must be identified. Second, the mutations must be analyzed in vivo at the cellular and molecular levels.
Third, those functional genes that have been missed by the two screens must be identified. The generation of transgenic zebrafish can provide useful tools for overcoming each of these challenges.
zebrafish hindbrain is likely derived from rhombomere 5 during early embryogenesis. Thus, by establishing a combinatorial genetic system for continuously reporting transient gene activity, we have laid the foundation for non-invasive spatiotemporal fate mapping and life-long continuous transgene expression in zebrafish.
ResultsCited by: This demonstrates that a tissue-specific promoter can reliably drive reporter gene expression in transgenic zebrafish in a manner identical to the control of the endogeneous expression of the gene.
Levels of GFP expression varied greatly from line to line; i.e., fluorescence was very weak in some lines, while it was extremely high in by: Analysis of reporter gene expression in transient and stable transgenic zebrafish embryos provides the opportunity for efficient and rapid detection of tissue-specific gene Cited by: Behavioral and Neural Genetics of Zebrafish assembles the state-of-the-art methodologies and current concepts pertinent to their neurobehavioral genetics.
Discussing their natural behavior, motor function, learning and memory, this book focuses on the fry and adult zebrafish, featuring a comprehensive account of modern genetic and neural. Efficient generation of knock-in transgenic zebrafish carrying reporter/ driver genes by CRISPR/Cas9-mediated genome engineering Yukiko Kimura1, Yu Hisano2, Atsuo Kawahara2,3 & Shin-ichi.Transgenic zebrafish have essentially had exogenous genes added to their genome and as such can be used for many different experimental applications such as stable overexpression, dominant-negative mutation expression, and general analysis of gene regulation.
Another common use is the expression of fluorescent proteins in cells of interest to mark promoter-specific tissues and changes in structure .In summary, we have described the developmental expression of a number of genes with relevance to OA in the zebrafish and we describe a new transgenic reporter line for Type X collagen, which can be used to study chondrocyte hypertrophy in live by: